An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes

@article{Harikrishna1996AnEG,
  title={An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes},
  author={Kulaveerasingam Harikrishna and Rachanee Jampates-Beale and Stephen B. Milligan and Charles S Gasser},
  journal={Plant Molecular Biology},
  year={1996},
  volume={30},
  pages={899-911}
}
A gene (pMON9617; Chi2;1) identified by screening a tomato pistil cDNA library has been found to encode a protein similar in sequence to class II chitinases. Using a baculovirus expression system we show that the Chi2;1 protein is an active endochitinase. The Chi2;1 protein is most similar in sequence to a previously described stylar chitinase (SK2) isolated from potato. Both proteins lack the diagnostic N-terminal cysteine-rich regions and the C-terminal vacuolar targeting signals of class I… CONTINUE READING

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