An endo-alpha1 leads to 6-D-mannanase from a soil bacterium. Purification, properties, and mode of action.

@article{Nakajima1976AnEL,
  title={An endo-alpha1 leads to 6-D-mannanase from a soil bacterium. Purification, properties, and mode of action.},
  author={Terumi Nakajima and S K Maitra and Clinton E. Ballou},
  journal={The Journal of biological chemistry},
  year={1976},
  volume={251 1},
  pages={174-81}
}
A soil organism, isolated by enrichment culture on unbranched alpha1 leads to 6-mannan backbone from the yeast Saccharomyces cerevisiae, secretes and endo-alpha1 leads to 6-mannanase. We have purified this mannanase to homogeneity and find it to consist of a single polypeptide chain with a molecular weight of about 131,000. The enzyme is unusually heat… CONTINUE READING