An empirical method for the estimation of nonspecific PCR primer derived genomic reactivity.

Abstract

A total of 36 PCR primers were subjected to thermal cycling, in a reaction composed of a single primer only, in order to assess their individual tendencies toward "nonspecific" amplification of human genomic DNA background. Nonspecific amplification of genomic DNA was estimated by Southern hybridization of the amplification products with an Alu-specific DNA… (More)

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