An electrogenic amino acid transporter in the apical membrane of cultured human bronchial epithelial cells.

@article{Galietta1998AnEA,
  title={An electrogenic amino acid transporter in the apical membrane of cultured human bronchial epithelial cells.},
  author={Luis J. V. Galietta and Luciana Musante and Leila Romio and Ubaldo Caruso and Annarita Fantasia and Andrea Gazzolo and Luca Roman{\`o} and Ospedale Luigi Sacco and Giovanni A Rossi and Luigi Varesio and Olga Zegarra-Moran},
  journal={American journal of physiology. Lung cellular and molecular physiology},
  year={1998},
  volume={275 5},
  pages={L917-L923}
}
We performed Ussing chamber experiments on cultured human bronchial epithelial cells to look for the presence of electrogenic dibasic amino acid transport. Apical but not basolaterall-arginine (10-1,000 μM) increased the short-circuit current. Maximal effect and EC50 were ∼3.5 μA/cm2 and 80 μM, respectively, in cells from normal subjects and cystic fibrosis patients. The involvement of nitric oxide was ruled out because a nitric oxide synthase inhibitor ( N G-nitro-l-arginine methyl ester) did… CONTINUE READING
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