An electrochemical assay of beta-1,3-glucanase gene from transgenic capsicum using asymmetric PCR.

@article{Wu2009AnEA,
  title={An electrochemical assay of beta-1,3-glucanase gene from transgenic capsicum using asymmetric PCR.},
  author={Guofan Wu and Zhihua Wang and Huini Zhang and Ning Yang and Jie Du and Xiao-Quan Lu and Jingwan Kang},
  journal={Nucleosides, nucleotides & nucleic acids},
  year={2009},
  volume={28 11},
  pages={1051-67}
}
5'-Thiol-derivatized specific DNA probes were added to the single primer polymerase chain reaction (asymmetric PCR) solution. In the PCR process, the DNA probes extended in the presence of target; the extended probes were then immobilized on a glassy carbon electrode (GCE) via gold nanoparticles. Finally, methylene blue and the extended probes were combined… CONTINUE READING