An efficient transformation method for Bacillus subtilis DB104

  title={An efficient transformation method for Bacillus subtilis DB104},
  author={Ljubica Vojcic and Dragana Despotovic and R. Mart{\'i}nez and K. Maurer and U. Schwaneberg},
  journal={Applied Microbiology and Biotechnology},
  • Ljubica Vojcic, Dragana Despotovic, +2 authors U. Schwaneberg
  • Published 2012
  • Biology, Medicine
  • Applied Microbiology and Biotechnology
  • Bacillus subtilis strains are used for extracellular expression of enzymes (i.e., proteases, lipases, and cellulases) which are often engineered by directed evolution for industrial applications. B. subtilis DB104 represents an attractive directed evolution host since it has a low proteolytic activity and efficient secretion. B. subtilis DB104 is hampered like many other Bacillus strains by insufficient transformation efficiencies (≤103 transformants/μg DNA). After investigating five physical… CONTINUE READING
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