An efficient transformation method for Bacillus subtilis DB104

@article{Vojcic2012AnET,
  title={An efficient transformation method for Bacillus subtilis DB104},
  author={Ljubica Vojcic and Dragana Despotovic and R. Mart{\'i}nez and K. Maurer and U. Schwaneberg},
  journal={Applied Microbiology and Biotechnology},
  year={2012},
  volume={94},
  pages={487-493}
}
  • Ljubica Vojcic, Dragana Despotovic, +2 authors U. Schwaneberg
  • Published 2012
  • Biology, Medicine
  • Applied Microbiology and Biotechnology
  • Bacillus subtilis strains are used for extracellular expression of enzymes (i.e., proteases, lipases, and cellulases) which are often engineered by directed evolution for industrial applications. B. subtilis DB104 represents an attractive directed evolution host since it has a low proteolytic activity and efficient secretion. B. subtilis DB104 is hampered like many other Bacillus strains by insufficient transformation efficiencies (≤103 transformants/μg DNA). After investigating five physical… CONTINUE READING
    39 Citations

    Figures, Tables, and Topics from this paper

    Explore Further: Topics Discussed in This Paper

    Fast genome editing in Bacillus subtilis
    • 1
    Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine
    • 16
    • Highly Influenced
    CopySwitch—in vivo Optimization of Gene Copy Numbers for Heterologous Gene Expression in Bacillus subtilis
    • 4
    • PDF
    Development of an Efficient Electroporation Method for Iturin A-Producing Bacillus subtilis ZK
    • 10
    • PDF
    Combined chemical and physical transformation method with RbCl and sepiolite for the transformation of various bacterial species.
    • 7
    • Highly Influenced
    A part toolbox to tune genetic expression in Bacillus subtilis
    • 81
    • PDF

    References

    SHOWING 1-10 OF 21 REFERENCES
    A rapid and simple method for Bacillus subtilis transformation on solid media.
    • 21
    • PDF
    Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism.
    • 400
    • PDF
    Genetic competence in Bacillus subtilis.
    • D. Dubnau
    • Biology, Medicine
    • Microbiological reviews
    • 1991
    • 370
    • Highly Influential
    • PDF
    Novel plasmid marker rescue transformation system for molecular cloning in Bacillus subtilis enabling direct selection of recombinants
    • 6
    • PDF
    High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA
    • 424
    • Highly Influential
    A Flow Cytometry–Based Screening System for Directed Evolution of Proteases
    • 40
    • Highly Influential
    DNA uptake in bacteria.
    • D. Dubnau
    • Biology, Medicine
    • Annual review of microbiology
    • 1999
    • 607
    • Highly Influential
    • PDF
    Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.
    • 285
    • Highly Influential
    • PDF