• DOI:10.1007/s00253-012-3987-2
Corpus ID: 1023560

An efficient transformation method for Bacillus subtilis DB104

@article{Vojcic2012AnET,
  title={An efficient transformation method for Bacillus subtilis DB104},
  author={Ljubica Vojcic and Dragana Despotovic and R. Mart{\'i}nez and K. Maurer and U. Schwaneberg},
  journal={Applied Microbiology and Biotechnology},
  year={2012},
  volume={94},
  pages={487-493}
}

Ljubica Vojcic, Dragana Despotovic, +2 authors U. Schwaneberg

Published 2012

Biology, Medicine

Applied Microbiology and Biotechnology

Bacillus subtilis strains are used for extracellular expression of enzymes (i.e., proteases, lipases, and cellulases) which are often engineered by directed evolution for industrial applications. B. subtilis DB104 represents an attractive directed evolution host since it has a low proteolytic activity and efficient secretion. B. subtilis DB104 is hampered like many other Bacillus strains by insufficient transformation efficiencies (≤103 transformants/μg DNA). After investigating five physical… CONTINUE READING

View on Springer

ncbi.nlm.nih.gov

Save to Library

Create Alert

Cite

Launch Research Feed