An efficient transfection method for mouse embryonic stem cells.

  title={An efficient transfection method for mouse embryonic stem cells.},
  author={J T Liou and Bor-Sheng Ko and Tzu-Ching Chang},
  journal={Methods in molecular biology},
Embryonic stem (ES) cells are an important source of stem cells in tissue engineering and regenerative medicine because of their high self-renewal capacities and differentiation potentials. However, the detailed molecular mechanisms controlling the differentiation and renewal programs in ES cells remained unclear. One of the difficulties in understanding these mechanisms substantially results from the low efficacies of gene manipulation by delivering exogenous gene expression or knockdown of… CONTINUE READING


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