An efficient technique for primer development and application that integrates fluorescent labeling and multiplex PCR1

@inproceedings{Culley2013AnET,
  title={An efficient technique for primer development and application that integrates fluorescent labeling and multiplex PCR1},
  author={Theresa M Culley and Trevor I. Stamper and Richard L. Stokes and Jessica R. Brzyski and Nicole A. Hardiman and Matthew R. Klooster and Benjamin J. Merritt},
  booktitle={Applications in plant sciences},
  year={2013}
}
UNLABELLED PREMISE OF THE STUDY Development of genetic markers can be costly and time-consuming, especially when multiple primer pairs are fluorescently labeled. This step was streamlined by combining two techniques in the same PCR reaction: (1) custom-labeling of primers by the investigator and (2) multiplexing multiple primers together in the same reaction. • METHODS AND RESULTS This technique was successfully used to develop microsatellite markers in several plant species… CONTINUE READING
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Isolation and characterization of microsatellite markers from Tiarella polyphylla (Saxifragaceae)

  • Jeong, M Masayuki, And J.-H Pak
  • American Journal of Botany
  • 2012
Highly Influential
5 Excerpts

AmplifX 1.6.1. Centre de Recherche en Neurobiologie et Neurophysiologie de Marseille (CNRS)

  • Jullien
  • AmplifX 1.6.1. Centre de Recherche en…
  • 2013

in Plant Sciences

  • in Plant Sciences
  • 2013

Development and characterization of microsatellite loci in Pericopsis elata (Fabaceae) using a cost-effi cient approach

  • Micheneau, G Dauby, N Bourland, J.-L Doucet, And O J Hardy
  • American Journal of Botany
  • 2011

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