An efficient strategy for the synthesis of circular RNA molecules.

@article{Beaudry1995AnES,
  title={An efficient strategy for the synthesis of circular RNA molecules.},
  author={Danielle Beaudry and J P Perreault},
  journal={Nucleic acids research},
  year={1995},
  volume={23 15},
  pages={3064-6}
}
We have developed a fast and simple one test-tube procedure for synthesizing large amounts of pure, homogeneous circular RNA molecules of any sequence. This new strategy is based on the exploitation of RNA internal secondary structure to position the 5' and 3' termini such that they will be single stranded, but held in close proximity to each other for subsequent ligation by T4 RNA ligase. This method consists of five simple steps (Fig. 1 A): (i) use of the circularly permuted RNA strategy (1… CONTINUE READING

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T 4RNA ligase

  • . D. Boyer, A. Sharp.P.
  • The Enzymes P
  • 1982

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