An efficient selection system for packaging herpes simplex virus amplicons.

@article{Zhang1998AnES,
  title={An efficient selection system for packaging herpes simplex virus amplicons.},
  author={Xufeng Zhang and Helen O'Shea and Claire Entwisle and Mike Boursnell and Stacey Efstathiou and Suzanne Inglis},
  journal={The Journal of general virology},
  year={1998},
  volume={79 ( Pt 1)},
  pages={125-31}
}
Due to their simplicity and flexibility of genomic construction, herpes simplex virus (HSV) amplicon-based vectors are attractive vehicles for gene delivery. However, a significant problem faced in the generation of amplicon stocks is the low amplicon to helper virus (A/H) ratio. In order to improve the proportion of amplicons generated, a selection system for amplicon production was developed in which the HSV thymidine kinase (TK) gene is inserted into an amplicon plasmid and an HSV mutant… CONTINUE READING