An efficient selection and regeneration protocol for Agrobacterium-mediated transformation of oriental melon (Cucumis melo L. var. makuwa)

@article{Choi2012AnES,
  title={An efficient selection and regeneration protocol for Agrobacterium-mediated transformation of oriental melon (Cucumis melo L. var. makuwa)},
  author={Jun Young Choi and Jeong Sheop Shin and Young Soo Chung and Nam-In Hyung},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2012},
  volume={110},
  pages={133-140}
}
An efficient selection and plant regeneration protocol for Agrobacterium-mediated transformation using cotyledon explants of oriental melon (Cucumis melo L. var. makuwa) has been developed. All six oriental melon cultivars evaluated in the study showed a >90 % shoot regeneration frequency and produced 1.8–3.6 shoots per cotyledon explant when cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg L−1 benzyladenine and 0.01 mg L−1 indoleacetic acid. Kanamycin (Km) and geneticin (Gt… CONTINUE READING
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