An efficient protein preparation for proteomic analysis of developing cotton fibers by 2-DE.

@article{Yao2006AnEP,
  title={An efficient protein preparation for proteomic analysis of developing cotton fibers by 2-DE.},
  author={Yuan Yao and Yi-wei Yang and Jinyuan Liu},
  journal={Electrophoresis},
  year={2006},
  volume={27 22},
  pages={4559-69}
}
Preparation of high-quality proteins from cotton fiber tissues is difficult due to high endogenous levels of polysaccharides, polyphenols, and other interfering compounds. To establish a routine procedure for the application of proteomic analysis to cotton fiber tissues, a new protocol for protein extraction was developed by optimizing a phenol extraction method combined with methanol/ammonium acetate precipitation. The protein extraction for 2-DE was remarkably improved by the combination of… CONTINUE READING

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