An efficient process for production of N-acetylneuraminic acid using N-acetylneuraminic acid aldolase.

@article{Mahmoudian1997AnEP,
  title={An efficient process for production of N-acetylneuraminic acid using N-acetylneuraminic acid aldolase.},
  author={M. Mahmoudian and D. Noble and C. Drake and R. F. Middleton and D. Montgomery and J. Piercey and D. Ramlakhan and M. Todd and M. Dawson},
  journal={Enzyme and microbial technology},
  year={1997},
  volume={20 5},
  pages={
          393-400
        }
}
N-acetyl-D-neuraminic acid (Neu5Ac) aldolase (EC 4.1.3.3) has bee reported for synthesis of Neu5Ac,1-5 but there are no reports of processes which do not have significant drawbacks for large-scale operation. Here, Neu5Ac aldolase from an overexpressing recombinant strain of Escherichia coli has been used to develop an immobilized enzyme process for production of Neu5Ac. The enzyme was immobilized onto Eupergit-C and could be reused many times in the reaction. Base-catalyzed epimerization of N… Expand
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