An efficient novel glycosylation of flavonoid by β-fructosidase resistant to hydrophilic organic solvents.

@article{Wu2013AnEN,
  title={An efficient novel glycosylation of flavonoid by β-fructosidase resistant to hydrophilic organic solvents.},
  author={Xueming Wu and Jianlin Chu and Bin Wu and Sen Zhang and Bingfang He},
  journal={Bioresource technology},
  year={2013},
  volume={129},
  pages={659-62}
}
An effective approach was successfully developed to isolate glycosidase with resistance of hydrophilic organic solvent, simultaneously with acceptor specificity of the target substrate. By this approach, an efficient solvent tolerant glycosidase producing bacterium Arthrobacter nicotianae XM6 was obtained. The β-fructosidase from strain XM6 shows high activity and stability in 10-25% DMSO and 10-20% methanol with 90-99% yields of puerarin glycosides. The addition of hydrophilic solvents not… CONTINUE READING