An efficient method to obtain axenic cultures of Alexandrium tamarense--a PSP-producing dinoflagellate.

@article{Su2007AnEM,
  title={An efficient method to obtain axenic cultures of Alexandrium tamarense--a PSP-producing dinoflagellate.},
  author={Jianqiang Su and Xiaoru Yang and Tianling Zheng and Huasheng Hong},
  journal={Journal of microbiological methods},
  year={2007},
  volume={69 3},
  pages={425-30}
}
The fact that species of harmful algae maintained in the laboratory harbor a complex bacterial flora increases the difficulties involved in the study of the relationship between bacteria and algae. An efficient method to remove bacteria from a laboratory culture of the marine dinoflagellate Alexandrium tamarense is presented in this paper. The alga was subjected to repeated washing, lysozyme/SDS and antibiotic treatment with a mixture of gentamycin, streptomycin, cephalothin and rifampicin… CONTINUE READING
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