An efficient method for the isolation of mycelial protoplasts from Coprinus macrorhizus and other basidiomycetes


A mixture of commercially available chitinase and cellulase released mycelial protoplasts of Coprinus macrorhizus in yields exceeding 108/ml plasts from C. macrorhizus FisC regenerated hyphae and developed into normal fruiting bodies at frequencies of 20%–50%. The same method also released good yields of protoplasts from several other edible mushroom… (More)
DOI: 10.1007/BF00252817


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