An efficient method for sonication assisted Agrobacterium-mediated transformation of coat protein (CP) coding genes into papaya (Carica papaya L.).

@article{Jiang2004AnEM,
  title={An efficient method for sonication assisted Agrobacterium-mediated transformation of coat protein (CP) coding genes into papaya (Carica papaya L.).},
  author={Ling Jiang and Tetsuo Maoka and Sadao Komori and Hiroshi Fukamachi and Hidenori Kato and Kazunori Ogawa},
  journal={Shi yan sheng wu xue bao},
  year={2004},
  volume={37 3},
  pages={189-98}
}
An efficient method for the production of transgenic papaya was developed via Sonication Assisted Agrobacterium-mediated Transformation (SAAT) of somatic embryos. The plasmid pGA482G was modified to contain gene PTi-Epj-TL-PLDMV with CP coding sequence of PLDMV Japan strain and chimeric gene PTi-NP-YKT with multiple CP coding sequences from PRSV Taiwan strain, PRSV Hawaii strain and PRSV Thailand strain, respectively. Disarmed Agrobacterium tumefaciens strain LBA4404 carrying the binary plasmid… CONTINUE READING
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