An efficient high-throughput flow cytometric method for estimating DNA ploidy level in plants.

@article{Cousin2009AnEH,
  title={An efficient high-throughput flow cytometric method for estimating DNA ploidy level in plants.},
  author={Anouska Cousin and Kathy Heel and Wallace A Cowling and Matthew N Nelson},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2009},
  volume={75 12},
  pages={1015-9}
}
We present an efficient high-throughput flow cytometric method that builds on previously published methods and permits rapid ploidy discrimination in plants. By using Brassica napus L. microspore-derived plants as an example, we describe how 192 leaf tissue samples may be processed and analyzed comfortably by one operator in 6 h from tissue sampling to ploidy determination. The technique involves placing young leaf samples in two 96-well racks, using a bead-beating procedure to release nuclei… CONTINUE READING
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