An efficient gene-disruption method in Cryptococcus neoformans by double-joint PCR with NAT-split markers.

Abstract

Targeted gene disruption via biolistic transformation and homologous recombination is a method widely used to identify and investigate the function of genes in Cryptococcus neoformans that causes fatal fungal meningitis if not timely treated. Currently, most laboratories employ the overlap-PCR method to generate a gene-disruption cassette with dominant… (More)
DOI: 10.1016/j.bbrc.2009.10.089

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Cite this paper

@article{Kim2009AnEG, title={An efficient gene-disruption method in Cryptococcus neoformans by double-joint PCR with NAT-split markers.}, author={Min Su Kim and Seo-Young Kim and Ja Kyung Yoon and Yin-Won Lee and Yong-Sun Bahn}, journal={Biochemical and biophysical research communications}, year={2009}, volume={390 3}, pages={983-8} }