An efficient extraction method to enhance analysis of low abundant proteins from soybean seed.

@article{Natarajan2009AnEE,
  title={An efficient extraction method to enhance analysis of low abundant proteins from soybean seed.},
  author={Savithiry S. Natarajan and Hari B Krishnan and Sukla Lakshman and Wesley M. Garrett},
  journal={Analytical biochemistry},
  year={2009},
  volume={394 2},
  pages={259-68}
}
Large amounts of the major storage proteins, beta-conglycinin and glycinin, in soybean (Glycine max) seeds hinder the isolation and characterization of less abundant seed proteins. We investigated whether isopropanol extraction could facilitate resolution of the low abundant proteins, different from the main storage protein fractions, in one-dimensional polyacrylamide gel electrophoresis (1D-PAGE) and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). 1D-PAGE of proteins extracted by… CONTINUE READING

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