An efficient culture method for generating large quantities of mature mouse splenic macrophages.

@article{Alatery2008AnEC,
  title={An efficient culture method for generating large quantities of mature mouse splenic macrophages.},
  author={Attiya Alatery and Sameh Basta},
  journal={Journal of immunological methods},
  year={2008},
  volume={338 1-2},
  pages={47-57}
}
In this study, we established an efficient in vitro culture method for generating mature splenic macrophages (Sp-Mphi). Splenocytes were cultured in the presence of conditioned medium containing macrophage colony-stimulating factor (M-CSF) for 7 days post post-isolation and the generated Sp-Mphi were characterized phenotypically and functionally. Through this method, 9 x 10(6)/mouse Sp-Mphi were obtained in comparison to 2 x 10(5)/mouse when Mphi were cultured in regular medium. In addition… CONTINUE READING
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