An efficient and high-throughput protocol for Agrobacterium-mediated transformation based on phosphomannose isomerase positive selection in Japonica rice (Oryza sativa L.)

@article{Duan2012AnEA,
  title={An efficient and high-throughput protocol for Agrobacterium-mediated transformation based on phosphomannose isomerase positive selection in Japonica rice (Oryza sativa L.)},
  author={Yongbo Duan and Chenguang Zhai and Hao Li and Juan Li and Wenqian Mei and Huaping Gui and Dahu Ni and Fengshun Song and Li Li and Wanggen Zhang and Jian-bo Yang},
  journal={Plant Cell Reports},
  year={2012},
  volume={31},
  pages={1611-1624}
}
A number of Agrobacterium-mediated rice transformation systems have been developed and widely used in numerous laboratories and research institutes. However, those systems generally employ antibiotics like kanamycin and hygromycin, or herbicide as selectable agents, and are used for the small-scale experiments. To address high-throughput production of transgenic rice plants via Agrobacterium-mediated transformation, and to eliminate public concern on antibiotic markers, we developed a… CONTINUE READING