An easy-to-run method for routine analysis of eumelanin and pheomelanin in pigmented tissues.

@article{Panzella2007AnEM,
  title={An easy-to-run method for routine analysis of eumelanin and pheomelanin in pigmented tissues.},
  author={Lucia Panzella and Paola Manini and Giuseppe Monfrecola and Marco d'Ischia and Alessandra Napolitano},
  journal={Pigment cell research},
  year={2007},
  volume={20 2},
  pages={128-33}
}
A procedure for analysis of melanin-pigmented tissues based on alkaline hydrogen peroxide degradation coupled with high-performance liquid chromatography (HPLC) ultraviolet determination of pyrrole-2,3,5-tricarboxylic acid (PTCA) for eumelanin and 6-(2-amino-2-carboxyethyl)-2-carboxy-4-hydroxybenzothiazole (BTCA) and 1,3-thiazole-2,4,5-tricarboxylic acid for pheomelanin was recently developed. Despite advantages related to the degradation conditions and sample handling, a decrease of the… CONTINUE READING

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