An easy ‘one tube’ method to estimate viability of Cryptosporidium oocysts using real-time qPCR

Abstract

Viability estimation of the highly resistant oocysts of Cryptosporidium remains a key issue for the monitoring and control of this pathogen. We present here a simple ‘one tube’ quantitative PCR (qPCR) protocol for viability estimation using a DNA extraction protocol which preferentially solubilizes excysted sporozoites rather than oocysts. Parasite DNA… (More)
DOI: 10.1007/s00436-016-5044-z

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@article{PaziewskaHarris2016AnE, title={An easy ‘one tube’ method to estimate viability of Cryptosporidium oocysts using real-time qPCR}, author={Anna Paziewska-Harris and Gerard J. Schoone and Henk D. H. F. Schallig}, journal={Parasitology Research}, year={2016}, volume={115}, pages={2873-2877} }