An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting

@inproceedings{Cao2016AnEA,
  title={An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting},
  author={Jian Cao and Lizhen Wu and Shang-Min Zhang and Min Lu and William K. C. Cheung and Wesley L. Cai and Molly Gale and Qi Xu and Qin Yan},
  booktitle={Nucleic acids research},
  year={2016}
}
The CRISPR/Cas9 system is a powerful genome editing tool and has been widely used for biomedical research. However, many challenges, such as off-target effects and lack of easy solutions for multiplex targeting, are still limiting its applications. To overcome these challenges, we first developed a highly efficient doxycycline-inducible Cas9-EGFP vector. This vector allowed us to track the cells for uniform temporal control and efficient gene disruption, even in a polyclonal setting… CONTINUE READING
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