An auxin-based degron system for the rapid depletion of proteins in nonplant cells

@article{Nishimura2009AnAD,
  title={An auxin-based degron system for the rapid depletion of proteins in nonplant cells},
  author={Kohei Nishimura and Tatsuo Fukagawa and Haruhiko Takisawa and Tatsuo Kakimoto and Masato T Kanemaki},
  journal={Nature Methods},
  year={2009},
  volume={6},
  pages={917-922}
}
Plants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUX/IAA family of transcription repressors by a specific form of the SCF E3 ubiquitin ligase. Other eukaryotes lack the auxin response but share the SCF degradation pathway, allowing us to transplant the auxin-inducible degron (AID) system into nonplant cells and use a small molecule to conditionally control protein stability. The AID system allowed rapid and reversible degradation of… CONTINUE READING
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