An automated technique for double staining rat and rabbit fetal skeletal specimens to differentiate bone and cartilage.

@article{Trueman1999AnAT,
  title={An automated technique for double staining rat and rabbit fetal skeletal specimens to differentiate bone and cartilage.},
  author={Daniel Trueman and Stanley W Jackson and brett. trueman},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  year={1999},
  volume={74 2},
  pages={98-104}
}
Assessment of chemicals for their potential to cause developmental toxicity must include evaluation of the development of the fetal skeleton. The method described here is an improved and fully automated double staining method using alizarin red S to stain bone and alcian blue to stain cartilage. The method was developed on the enclosed Shandon Pathcentre, and the quality of specimens reported here will be reproduced only if carried out on a similar processor under the same environmental… CONTINUE READING

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