An automated assay of the behavioral effects of cocaine injections in adult Drosophila

  title={An automated assay of the behavioral effects of cocaine injections in adult Drosophila
  author={Nikola Dimitrijevi{\'c} and Svetlana Dzitoyeva and Hari Manev},
  journal={Journal of Neuroscience Methods},
High Throughput Measurement of Locomotor Sensitization to Volatilized Cocaine in Drosophila melanogaster
FlyBong is a new and improved method for inducing and measuring locomotor sensitization to cocaine in individual Drosophila, and it is validated by showing that locomot sensitization at either the population or individual level is absent in the mutants for circadian genes period, Clock, and cycle.
Genetics of Cocaine and Methamphetamine Consumption and Preference in Drosophila melanogaster
Results suggest that variation in consumption and development of preference for both cocaine and methamphetamine is mediated, at least in part, through a neural network that comprises dopaminergic projections to the mushroom bodies.
The Use of Drosophila to Understand Psychostimulant Responses
How Drosophila is an efficient and cost-effective model organism for identifying novel candidate genes and molecular mechanisms involved in the behavioral responses to psychostimulant drugs is discussed.
Cocaine Tolerance in Honey Bees
The authors' data show clear short and long-term behavioural responses of bees to cocaine administration, but caution that, despite the small size of the bee brain, measures of biogenic amines conducted at the whole-brain level may not reveal neurochemical effects of the drug.
Drosophila melanogaster as a model to study drug addiction
The advantages of using the fly to study drug-related behavior, the molecular mechanisms and neural circuits underlying drug-induced behavior in flies are described, and a brief overview of the behavioral assays used are provided.
First evidence that drugs of abuse produce behavioral sensitization and cross sensitization in planarians
Demonstration of pharmacologically selective behavioral sensitization in planarians suggests that these flatworms represent a sensitive in-vivo model to study cocaine Behavioral sensitization and to screen potential abuse-deterrent therapeutics.
GABA-B receptors in Drosophila.


Cocaine sensitization and reward are under the influence of circadian genes and rhythm
It is demonstrated that processes involved in cocaine addiction depend on the circadian rhythm and are modulated in an opposing manner by mPer1 and mPer2 genes.
Activating properties of cocaine and cocaethylene in a behavioral preparation of Drosophila melanogaster
The receptor protein mediating the behavioral responses to stimulant drugs in Drosophila is pharmacologically similar to the mammalian D1 subtype, as in rats, and the NMDA (and D1) receptor pathways in this arthropod represent obligatory targets for the behavioral effects of stimulants.
Invertebrate models of drug abuse.
Recent behavioral and genetic studies in flies and worms on the effects of ethanol, cocaine, and nicotine, three of the most widely abused drugs in the world are reviewed.
γ-Aminobutyric acid B receptor 1 mediates behavior-impairing actions of alcohol in Drosophila: Adult RNA interference and pharmacological evidence
The Drosophila model the authors have developed can be used for further in vivo functional characterization of GABAB receptor subunits and their involvement in the molecular and systemic actions of addictive substances.
Requirement of circadian genes for cocaine sensitization in Drosophila.
Findings indicate unexpected roles for these genes in regulating cocaine sensitization and indicate that they function as regulators of tyrosine decarboxylase.
The Pineal Gland is Critical for Circadian Period1 Expression in the Striatum and for Circadian Cocaine Sensitization in Mice
The results indicate that both the striatal circadian Per1 expression and diurnal locomotor cocaine sensitization are strongly influenced by pineal products, and it is suggested that further studies on pineal-driven mechanisms will help to understand the mechanisms of drug abuse and identify novel targets for the prevention and/or treatment of addictions.