An automated approach for three-dimensional quantification of fibrillar structures in optically cleared soft biological tissues.

@article{Schriefl2013AnAA,
  title={An automated approach for three-dimensional quantification of fibrillar structures in optically cleared soft biological tissues.},
  author={Andreas J{\"o}rg Schriefl and Heimo Wolinski and P. Regitnig and Sepp D. Kohlwein and Gerhard A Holzapfel},
  journal={Journal of the Royal Society, Interface},
  year={2013},
  volume={10 80},
  pages={20120760}
}
We present a novel approach allowing for a simple, fast and automated morphological analysis of three-dimensional image stacks (z-stacks) featuring fibrillar structures from optically cleared soft biological tissues. Five non-atherosclerotic tissue samples from human abdominal aortas were used to outline the multi-purpose methodology, applicable to various tissue types. It yields a three-dimensional orientational distribution of relative amplitudes, representing the original collagen fibre… CONTINUE READING