An assessment of the importance of exposure routes to the uptake and internal localisation of fluorescent nanoparticles in zebrafish (Danio rerio), using light sheet microscopy

@article{Skjolding2017AnAO,
  title={An assessment of the importance of exposure routes to the uptake and internal localisation of fluorescent nanoparticles in zebrafish (Danio rerio), using light sheet microscopy},
  author={Lars Michael Skjolding and Giedrė A{\vs}monaitė and Rasmus I. J{\o}lck and Thomas Lars Andresen and Henriette Selck and Anders Baun and Joachim Sturve},
  journal={Nanotoxicology},
  year={2017},
  volume={11},
  pages={351 - 359}
}
Abstract A major challenge in nanoecotoxicology is finding suitable methods to determine the uptake and localisation of nanoparticles on a whole-organism level. Some uptake methods have been associated with artefacts induced by sample preparation, including staining for electron microscopy. This study used light sheet microscopy (LSM) to define the uptake and localisation of fluorescently labelled nanoparticles in living organisms with minimal sample preparation. Zebrafish (Danio rerio) were… 

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