An assessment of the importance of exposure routes to the uptake and internal localisation of fluorescent nanoparticles in zebrafish (Danio rerio), using light sheet microscopy

@article{Skjolding2017AnAO,
  title={An assessment of the importance of exposure routes to the uptake and internal localisation of fluorescent nanoparticles in zebrafish (Danio rerio), using light sheet microscopy},
  author={L. Skjolding and G. A{\vs}monaitė and R. I. J{\o}lck and T. Andresen and H. Selck and A. Baun and J. Sturve},
  journal={Nanotoxicology},
  year={2017},
  volume={11},
  pages={351 - 359}
}
Abstract A major challenge in nanoecotoxicology is finding suitable methods to determine the uptake and localisation of nanoparticles on a whole-organism level. Some uptake methods have been associated with artefacts induced by sample preparation, including staining for electron microscopy. This study used light sheet microscopy (LSM) to define the uptake and localisation of fluorescently labelled nanoparticles in living organisms with minimal sample preparation. Zebrafish (Danio rerio) were… Expand
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References

SHOWING 1-10 OF 53 REFERENCES
Impact of dietary gold nanoparticles in zebrafish at very low contamination pressure: The role of size, concentration and exposure time
TLDR
Investigation of the impact of a daily ration of food containing gold nanoparticles in the zebrafish Danio rerio showed alteration of genome composition using a RAPD-PCR genotoxicity test as the number of hybridization sites of the RAPD probes was significantly modified after AuNP exposure. Expand
Quantification of gold nanoparticle cell uptake under controlled biological conditions and adequate resolution.
TLDR
The importance of studying NP-cell interactions under controlled experimental conditions and at adequate microscopic resolution in combination with stereology is emphasized, suggesting a predominant uptake via caveolin-medicated endocytois. Expand
Toxicity of silver nanoparticles in zebrafish models.
TLDR
The results suggest that silver nanoparticles induce a dose-dependent toxicity in embryos, which hinders normal development. Expand
Ingestion of metal-nanoparticle contaminated food disrupts endogenous microbiota in zebrafish (Danio rerio).
TLDR
Unique changes in zebrafish microbiome caused by exposure to Ag-NP and Cu-NP indicate that NP ingestion could affect digestive system function and organism health. Expand
Impact of gold nanoparticles on zebrafish exposed to a spiked sediment
TLDR
Gold accumulation in fish tissues demonstrated the lower bioavailability of AuNP compared to ionic Au, and underlined the higher toxic potential of the nanoparticle form. Expand
Evidence of one-way flow bioaccumulation of gold nanoparticles across two trophic levels
This work reports a one-way flow bioaccumulation of gold nanoparticles (AuNPs) in aquatic organisms between two trophic levels. First, Dunaliella salina cells were exposed to citrate-capped AuNPs atExpand
Silver nanoparticles: behaviour and effects in the aquatic environment.
TLDR
The ecotoxicological literature shows that concentrations of Ag NPs below the current and future PECs, as low as just a few ng L(-1), can affect prokaryotes, invertebrates and fish indicating a significant potential, though poorly characterised, risk to the environment. Expand
Behavioural toxicity assessment of silver ions and nanoparticles on zebrafish using a locomotion profiling approach.
TLDR
It was concluded that the locomotion-based behaviour-testing scheme can be used jointly with FET and can provide endpoints for sub-lethal toxicity testing within the FET framework. Expand
Persistent adult zebrafish behavioral deficits results from acute embryonic exposure to gold nanoparticles.
TLDR
It is demonstrated that acute, developmental exposure to 1.5 nm MES- and TMAT-AuNPs, two NPs differing only in the functional group, affects larval behavior, with behavioral effects persisting into adulthood. Expand
Silver nanoparticles affect the neural development of zebrafish embryos
TLDR
It is suggested that AgNPs could affect the neural development of zebrafish embryos, and the toxicity of AgNps may be partially attributed to the comparatively higher uptake in the head area. Expand
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5
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