An assay to quantify vascular colonization of potato byVerticillium dahliae

@article{Hoyos2008AnAT,
  title={An assay to quantify vascular colonization of potato byVerticillium dahliae},
  author={German P. Hoyos and Paul J. Zambino and Neil A. Anderson},
  journal={American Potato Journal},
  year={2008},
  volume={68},
  pages={727-742}
}
A procedure was developed and tested to evaluate potato germ plasm for relative rates of vascular colonization byVerticillium dahliae. The number of colony forming units (CFU) per ml of plant sap, extracted from stems and plated on a medium, was used to assess vascular colonization from plants grown for 100 days in a field plot infested withV. dahliae. Sap extracted from main stem tissue at the ground line gave more CFU ofV. dahliae than tissue at the mid and apical region of the main stem… CONTINUE READING
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