An assay for peptide binding to HLA-Cw*0102.

  title={An assay for peptide binding to HLA-Cw*0102.},
  author={Mads Hald Andersen and Ib S\ondergaard and Jesper Zeuthen and Tim Elliott and John S. Haurum},
  journal={Tissue antigens},
  volume={54 2},
The assembly assay for peptide binding to class I major histocompatibility complex (MHC) molecules is based on the ability of peptides to stabilize MHC class I molecules synthesized by transporter associated with antigen processing (TAP)-deficient cell. The TAP-deficient cell line T2 has previously been used in the assembly assay to analyze peptide binding to HLA-A*0201 and -B*5101. In this study, we have extended this technique to assay for peptides binding to endogenous HLA-Cw*0102 molecules… CONTINUE READING