An assay for local quality in cryo-electron micrographs of single particles.

@article{Gao2002AnAF,
  title={An assay for local quality in cryo-electron micrographs of single particles.},
  author={Haixiao Gao and Christian Spahn and Robert A. Grassucci and Joachim Frank},
  journal={Ultramicroscopy},
  year={2002},
  volume={93 2},
  pages={169-78}
}
High quality of the cryo-electron micrographs is of crucial importance for the success of single particle three-dimensional reconstruction methods. In analyzing some micrographs from cryo-electron microscopy specimens, we found an extraordinary variability, within the same micrograph, in the appearance of particles. We developed a method for analyzing the variability of local image quality, using correspondence analysis of local power spectra. With this technique, we discovered a strong… CONTINUE READING

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