An ascorbate-dependent artifact that interferes with the interpretation of the biotin switch assay.

Abstract

As an example of an important redox-based protein posttranslational modification, protein S-nitrosation of specific cysteines is attracting more and more attention. The methods of detecting protein S-nitrosation in vitro or in vivo have been widely used in recent research, especially the biotin switch assay. An increase in band intensity in the presence of… (More)

Topics

Cite this paper

@article{Huang2006AnAA, title={An ascorbate-dependent artifact that interferes with the interpretation of the biotin switch assay.}, author={Bo Huang and Chang Chen}, journal={Free radical biology & medicine}, year={2006}, volume={41 4}, pages={562-7} }