An archaebacterial 5S rRNA contains a long insertion sequence

  title={An archaebacterial 5S rRNA contains a long insertion sequence},
  author={Kenneth R. Luehrsen and Diarmuid E. Nicholson and David C. Eubanks and George E. Fox},
Sequence characterization of 16S rRNA has revealed an ancient divergence that divides prokaryotic organisms into eubacteria and archaebacteria1. This evolutionary split is so fundamental that by the 16S rRNA criterion, the archaebacteria appear to be as distinct from eubacteria as they are from eukaryotes2. Extensive comparison of the lipids3 and cell walls4,5 of the various archaebacterial species strongly supports this interpretation. Detailed molecular investigations of the archaebacteria… 

Initiator tRNAs from archaebacteria show common unique sequence characteristics

The nucleotide sequences of initiator tRNAs from Sulfolobus acidocaldarius, Halococcus morrhuae and Thermoplasma acidophilum are determined, and it is found that they have a common unique structural feature that differs from both true bacterial initiatortRNAs and eukaryotic initiatorTRNAs.

Characterisation of a novel pathway for ribosomal RNA maturation in Sulfolobus acidocaldarius

The aim of this study is to analyze possible novel regulatory mechanisms in the rRNA genes of the extremely thermoacidophilic archaebacterium Sulfolobus acidoccddccrius to discover rRNAgene regulation, transcript processing and requirements for mature ribosome function.

Expansion Segments in Bacterial and Archaeal 5S Ribosomal RNAs

The results of a comprehensive search for the expansion sequences in prokaryotic 5S rRNAs of 15 structural types were identified in 15 archaeal and 36 bacterial genomes, with results indicating that several strains harboring the expanded sequences belong to the bacterial orders Clostridiales, Halanaerobiale, ThermoanaerOBacterales, and Alteromonadales.

Gene structure, organization, and expression in archaebacteria.

The structure of protein and stable RNA-encoding genes cloned and sequenced from each of the major classes of archaebacteria: the methanogens, extreme halophiles, and acid thermophiles are compared.

Cryo-Electron Microscopy Visualization of a Large Insertion in the 5S ribosomal RNA of the Extremely Halophilic Archaeon Halococcus morrhuae

A cryo-electron microscopy reconstruction of the native large subunit of the extreme halophile Halococcus morrhuae (ATCC® 17082) shows that the insertion site forms a four-way junction that fully preserves the canonical 5S rRNA structure.

Structure, function and evolution of 5-S ribosomal RNAs.



Stable large variant of 5S RNA in Clostridium thermosaccharolyticum

The precursor forms of prokaryote 16S and 23S ribosomal RNA (rRNA) are larger than their mature counterparts by some 10% (refs 1–5), and the discovery of a stable form of the 5S RNA some 30–40% larger than normal, in Clostridium thermosaccharolyticum.

Nucleotide sequences of Acanthamoeba castellanii 5S and 5.8S ribosomal ribonucleic acids: phylogenetic and comparative structural analyses.

This sequence does not fit parts of existing secondary structure models for 5.8S rRNA, and the significance of such models is questioned.

Archaebacterial elongation factor is ADP-ribosylated by diphtheria toxin

It is reported here that diphtheria toxin also catalyses the ADP-ribosylation of archaebacterial elongation factors, meaning that these factors have to be assigned to the EF2 type; it is assumed that the ADp- ribosylatable structure arising so early in evolution is of fundamental importance for the elongation process.

Evolutionary relationship between Halobacterium cutirubrum and eukaryotes determined by use of aminoacyl-tRNA synthetases as phylogenetic probes.

Results suggest that phylogenetically H. cutirubrum is more closely related to the eukaryotes than to the E. coli or R. spheroides, and that prokaryotes related to these two groups are more related than to each other.

The nucleotide sequence of the 5S rRNA from the archaebacterium Thermoplasma acidophilum.

Results give further support to the notion that the archaebacteria represent an extremely early divergence among entities with procaryotic organization.

Phylogenetic structure of the prokaryotic domain: The primary kingdoms

  • C. WoeseG. Fox
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1977
A phylogenetic analysis based upon ribosomal RNA sequence characterization reveals that living systems represent one of three aboriginal lines of descent: the eubacteria, comprising all typical bacteria, the archaebacteria, and the urkaryotes, now represented in the cytoplasmic component of eukaryotic cells.

Secondary structure of eukaryotic cytoplasmic 5S ribosomal RNA.

  • K. LuehrsenG. Fox
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
A five-helix secondary structural model is proposed for eukaryotic cytoplasmic 5S rRNA. All available sequence data are consistent with this model including those from Chlorella 5S rRNA whose

Use of T4 RNA ligase to construct model substrates for a ribosomal RNA maturation endonuclease.

The susceptibilities of the artificial substrates to RNase M5 demonstrate that the symmetrically arranged sequences are not used in the RN enzyme M5 interaction with the precursor, and the synthetic protocols permitted the invention of an acid-soluble assay for RNaseM5 and, potentially, other specific endoribonucleases.

5S RNA secondary structure

It is possible that functional conformations of the 5SRNA molecule need only exist in the context of the ribosome, and consequently, may only exist therein, and the interpretation of experimental investigations on the isolated 5S RNA molecule may have inherent difficulties.