An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine.

Abstract

In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in immature loblolly pine (Pinus taeda) zygotic and somatic embryos, but is undetectable in later-stage embryos, megagametophytes, and roots, stems, and needles from 1 year-old seedlings. Analysis of PtNIP1;1 transcript in embryo proper and suspensor tissues by reverse transcription-polymerase chain reaction suggests preferential expression in the suspensor. Based on comparisons of derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like members of the major intrinsic protein superfamily branch of the aquaporin (major intrinsic protein) superfamily. Through heterologous expression in Xenopus laevis oocytes and the yeast (Saccharomyces cerevisiae) fps1(-) mutant, PtNIP1;1 has been shown to be an active aquaglyceroporin.

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@article{Ciavatta2001AnAI, title={An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine.}, author={Vincent T. Ciavatta and R. Morill{\'o}n and Gerald S . Pullman and M J Chrispeels and John Cairney}, journal={Plant physiology}, year={2001}, volume={127 4}, pages={1556-67} }