An application of PCR-single strand conformation polymorphism to MN genotyping.

@article{Sasaki2000AnAO,
  title={An application of PCR-single strand conformation polymorphism to MN genotyping.},
  author={Yoshihiro Sasaki and Nori Nakayashiki and Kiyoshi Saigusa and Masataka Takamiya and Yasuhiro Aoki},
  journal={Legal medicine},
  year={2000},
  volume={2 3},
  pages={171-4}
}
A PCR-based genotyping of MN blood group system was investigated for DNA samples taken from a population of 409 northern Japanese. DNA fragment (257bp) including exon 2 of glycophorin A (GPA) gene, in which encodes the determinants of MN antigens, was specifically amplified. On the analysis of PCR-single-strand conformation polymorphism (PCR-SSCP) for M alleles, band patterns of M(G) and M(T) were easily discriminated each other. For N alleles, three band patterns were observed, and we… CONTINUE READING

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