An antiviral RISC isolated from Tobacco rattle virus-infected plants.

@article{Ciomperlik2011AnAR,
  title={An antiviral RISC isolated from Tobacco rattle virus-infected plants.},
  author={Jessica J. Ciomperlik and Rustem T Omarov and Herman B Scholthof},
  journal={Virology},
  year={2011},
  volume={412 1},
  pages={
          117-24
        }
}
The RNAi model predicts that during antiviral defense a RNA-induced silencing complex (RISC) is programmed with viral short-interfering RNAs (siRNAs) to target the cognate viral RNA for degradation. We show that infection of Nicotiana benthamiana with Tobacco rattle virus (TRV) activates an antiviral nuclease that specifically cleaves TRV RNA in vitro. In agreement with known RISC properties, the nuclease activity was inhibited by NaCl and EDTA and stimulated by divalent metal cations; a novel… CONTINUE READING

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