An antibody with Fab-constant domains exchanged for a pair of CH3 domains

@inproceedings{WozniakKnopp2018AnAW,
  title={An antibody with Fab-constant domains exchanged for a pair of CH3 domains},
  author={Gordana Wozniak-Knopp and Gerhard Stadlmayr and Jan Walther Perthold and Katharina Stadlbauer and Mathias Gotsmy and Stefan Becker and Florian R{\"u}ker},
  booktitle={PloS one},
  year={2018}
}
We have designed a complete antibody-like construct where the CH1 and Cκ domains are exchanged for a pair of the CH3 domains and efficient pairing of the heavy and light variable domain is achieved using "Knobs-into-Holes" strategy. This construct, composed of only naturally occurring immunoglobulin sequences without artificial linkers, expressed at a high level in mammalian cells, however exhibited low solubility. Rational mutagenesis aimed at the amino acid residues located at the interface… CONTINUE READING