During a 20-year survey we routinely collected Tabanidae in the Sinai resulting in a checklist of 22 species. We recorded 2 species for the Levant (Tabanus mordax Austen, 1911 and Tabanus gratus Loew, 1858), 4 species for Egypt (Nemorius irritans (Ricardo, 1901), Chrysops flavipes Meigen, 1804, Haematopota coronata Austen, 1908 and Haematopota pallens Loew, 1871), 5 species for the Sinai (Atylotus farinosus (Szilády, 1915), Tabanus arenivagusAusten, 1920, Tabanus autumnalis Linnaeus, 1761, Haematopota minusculaAusten, 1920 and Dasyrhamphis nigritus Fabricius, 1794) for the first time and confirmed one doubtful record for the Sinai (Tabanus albifacies Loew, 1856). Furthermore we collected 10 more species that were previously known or could be presumed from the Sinai. The status of 4 species (Atylotus pallescens (Walker, 1871), Tabanus unifasciatus Loew, 1858, Tabanus politus (Walker, 1871) and Tabanus terminalis Walker, 1871) doubtfully recorded from the Sinai is discussed. For most of the 22 species, apart from zoogeographical notes, host and ecological observations are also given.