An analytical method for the detection of methylation differences at specific chromosomal loci using primer extension and ion pair reverse phase HPLC

@article{Matin2002AnAM,
  title={An analytical method for the detection of methylation differences at specific chromosomal loci using primer extension and ion pair reverse phase HPLC},
  author={Maryam M. Matin and Alessandra Baumer and David P Hornby},
  journal={Human Mutation},
  year={2002},
  volume={20}
}
We have developed a rapid, accurate, and quantitative method for the detection of methylation differences at specific CpG sites based on bisulfite treatment of DNA followed by primer extension and ion‐pair reversed‐phase high performance liquid chromatography (IP RP HPLC). The application of the method is illustrated by analysis of differentially imprinted alleles arising from Prader‐Willi and Angelman syndromes. In order to convert unmethylated cytosines to uracil, plasmid and genomic DNA… 

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