An analysis of the sensitivity and specificity of the cytokeratin marker CAM 5.2 for epithelial tumours. Results of a study of 203 sarcomas, 50 carcinomas and 28 malignant melanomas

@article{Leader1986AnAO,
  title={An analysis of the sensitivity and specificity of the cytokeratin marker CAM 5.2 for epithelial tumours. Results of a study of 203 sarcomas, 50 carcinomas and 28 malignant melanomas},
  author={Mary B Leader and Jay Patel and Carol A. Makin and Kristin Henry},
  journal={Histopathology},
  year={1986},
  volume={10}
}
Two hundred and three sarcomas, 40 carcinomas, 10 carcinomas with spindle cell features, 27 malignant melanomas and one spindle cell melanoma were examined using CAM 5.2, a monoclonal antibody to cytokeratin. This antibody which was prepared against colorectal carcinoma cells and which identifies low molecular weight intermediate filament cytokeratin proteins is suitable for use in formalin fixed, paraffin embedded material. Seventeen of the 203 sarcomas showed positive staining. These included… 
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KP-1: not a specific marker. Staining of 137 sarcomas, 48 lymphomas, 28 carcinomas, 7 malignant melanomas and 8 cystosarcoma phyllodes
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A combination of immunostains, including GFAP and cytokeratin CAM5.2, may be the most useful in differentiating poorly differentiated metastatic carcinoma from GBM.
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The results show this antibody to be a sensitive and specific marker of mesenchymal derivation or differentiation and a useful tool in separating sarcomas from most carcinomas, and in separating malignant melanomas from carcinomas.
Expression of the cytokeratin marker CAM 5.2 in cervical neoplasia
TLDR
Although CAM 5.2 expression is found in only 28% of cervical squamous carcinoma, it is highly specific for malignant transformation of cervical Squamous epithelium and needs to be examined in other laboratories under various processing schedules.
Utility of Immunohistochemistry in Separating Thymic Neoplasms From Germ Cell Tumors and Metastatic Lung Cancer Involving the Anterior Mediastinum
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An IHC panel consisting of CD5, CK5/6, PLAP, TTF-1, and CD57 is useful for the separation of thymic neoplasms from other epithelial malignancies involving the mediastinum.
MAC387: its non‐specificity as a tumour marker or marker of histiocytes
TLDR
The results of this study suggest that Mac387 is of little value in the differential diagnosis of soft tissue tumours, nor is it a specific histiocytic marker.
Cytokeratins in cervical dysplasia and neoplasia: A comparative study of immunohistochemical staining using monoclonai antibodies NCL‐5D3, CAM 5·2, and PKK1
TLDR
A study of 127 cases confirms the expression of low molecular weight cytokeratins in intraepithelial neoplasia of grade CIN III or greater in cervical biopsies and compares the use of three monoclonal antibodies in demonstrating the phenomenon.
Cytokeratin immunoreactivity in gliomas
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The immunostaining of astrocytomas and oligodendrogliomas with AE1/3 might represent co‐expression of cytokeratin with glial fibrillary acidic protein by gliomas and calls for caution in the use of these antibodies in the differential diagnosis between glioma and carcinomas.
Comparison of commercially available cytokeratin antibodies in normal and neoplastic adult epithelial and non-epithelial tissues.
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Five commercially available cytokeratin antibodies were used to stain a wide range of normal and neoplastic epithelial and non-epithelial tissues to assess their potential value in diagnostic histopathology, and all showed good specificity, with some cross-reactivity in smooth muscle cells.
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