An amphitropic cAMP-binding protein in yeast mitochondria. 2. Phospholipid nature of the membrane anchor.

Abstract

We describe the first example of a mitochondrial protein with a covalently attached phosphatidylinositol moiety acting as a membrane anchor. The protein can be metabolically labeled with both stearic acid and inositol. The stearic acid label is removed by phospholipase D whereupon the protein with the retained inositol label is released from the membrane. This protein is a cAMP receptor of the yeast Saccharomyces cerevisiae and tightly associated with the inner mitochondrial membrane. However, it is converted into a soluble form during incubation of isolated mitochondria with Ca2+ and phospholipid (or lipid derivatives). This transition requires the action of a proteinaceous, N-ethylmaleimide-sensitive component of the intermembrane space and is accompanied by a decrease in the lipophilicity of the cAMP receptor. We propose that the component of the intermembrane space triggers the amphitropic behavior of the mitochondrial lipid-modified cAMP-binding protein through a phospholipase activity.

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Cite this paper

@article{Mller1989AnAC, title={An amphitropic cAMP-binding protein in yeast mitochondria. 2. Phospholipid nature of the membrane anchor.}, author={G{\"{u}nter M{\"{u}ller and Wolfhard Bandlow}, journal={Biochemistry}, year={1989}, volume={28 26}, pages={9968-73} }