An amperometric biosensor with human CYP3A4 as a novel drug screening tool.

@article{Joseph2003AnAB,
  title={An amperometric biosensor with human CYP3A4 as a novel drug screening tool.},
  author={Shiba Joseph and James F. Rusling and Yuri Lvov and Thomas Friedberg and Uwe Fuhr},
  journal={Biochemical pharmacology},
  year={2003},
  volume={65 11},
  pages={1817-26}
}
We developed a biosensor based on the redox properties of human CYP3A4 to directly monitor electron transfer to the heme protein. Enzyme films were assembled on gold electrodes by alternate adsorption of a CYP3A4 layer on top of a polycation layer. Direct, reversible electron transfer between the electrode and CYP3A4 was observed with voltammetry under anaerobic conditions. In the presence of oxygen, the oxidation peak of the hemoprotein disappeared, and the reduction peak increased 2- to 3… CONTINUE READING