An amazing sequence arrangement at the 5′ ends of adenovirus 2 messenger RNA

@article{Chow1977AnAS,
  title={An amazing sequence arrangement at the 5′ ends of adenovirus 2 messenger RNA},
  author={Louise T. Chow and Richard Gelinas and Thomas R. Broker and Richard J. Roberts},
  journal={Cell},
  year={1977},
  volume={12},
  pages={1-8}
}
The 5' terminal sequences of several adenovirus 2 (Ad2) mRNAs, isolated late in infection, are complementary to sequences within the Ad2 genome which are remote from the DNA from which the main coding sequence of each mRNA is transcribed. [...] Key Result The 5' terminal sequences of mRNAs in R loops, variously located between positions 36 and 92, form complex secondary hybrids with single-stranded DNA from restriction endonuclease fragments containing sequences to the left of position 36 on the Ad2 genome.Expand
Sequences from the beginning of the fiber messenger RNA of adenovirus-2.
Small restriction fragments, from around co-ordinate 86.6 on the adenovirus-2 genome, have been used as primers for direct DNA sequence analysis by Sanger's (Sanger et al., 1977) chain terminationExpand
Complex splicing patterns of RNAs from the early regions of adenovirus-2.
TLDR
From the large, complex arrays of composite RNA structures, numerous insights into the RNA splicing mechanisms were inferred. Expand
Definition of a novel promoter for the major adenovirus-associated virus mRNA
TLDR
Combined DNA and RNA sequence analyses strongly suggest that the major polysomal RNA may be generated from a transcription unit with a promoter at position 39, even though this transcription unit is part of a larger transcriptionunit with an upstream promoter near position 6. Expand
RNA structures near poly(A) of adenovirus-2 late messenger RNAs.
TLDR
RNA sequences near poly(A) of adenovirus-2 late messenger RNAs have been mapped within the Ad2 genome and compared by T 1 ribonuclease fingerprinting and a hypothesis for the role of 3′ terminus formation in Ad2 mRNA synthesis is proposed. Expand
The spliced structures of adenovirus 2 fiber message and the other late mRNAs
TLDR
The number of RNA species transcribed from the Ad2 chromosome between coordinates 29 and 50 exceeds the number of identified Ad2 proteins, and the diversity of splicing patterns and the variety of recombined sequences generated during the synthesis of late Ad2 mRNAs are emphasized. Expand
Sequence analysis of adenovirus DNA. IV. The genomic sequences encoding the common tripartite leader of late adenovirus messenger RNA.
TLDR
The regions on the adenovirus type 2 genome which encode the three segments of the common tripartite leader of late adenvirus messenger RNA have been identified and sequenced and exhibit a striking similarity to the corresponding regions of eukaryotic genes. Expand
Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA
TLDR
This prototype sequence, which has also been recognized at or near the splice points in other eucaryotic systems, is possibly part of a larger unit which serves as a recognition site for specific excision-ligation events that ultimately lead to the production of mature mRNAs. Expand
Structure and metabolism of adenovirus RNAs containing sequences from the fiber gene.
TLDR
This study identified and characterized polyadenylated nuclear RNAs that contain fiber sequences and therefore are possible processing intermediates for adenovirus fiber messenger RNA and results are consistent with a precursor function for the 35 S RNAs and the 85.9 to 96.9 28 S species. Expand
Coincidence of the promoter and capped 5′ terminus of RNA from the adenovirus 2 major late transcription unit
TLDR
Strong homologies at cap sites and splice points suggest that for the noted cases, the virus and cell share closely related mechanisms for mRNA 5' end synthesis and splicing. Expand
A supplementary adenoviral leader sequence and its role in messenger translation
TLDR
Fractionation of total poly(A)-containing Ad2 + ND1 dp2 "late" cytoplasmic RNA in methyl mercuric hydroxide-agarose gels established that the presence or absence of the fourth leader sequence has little or no influence on the ability of the messenger to direct the synthesis of the 23–24K dalton protein(s) in a cell-free translation system derived from rabbit reticulocytes. Expand
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TLDR
Observations suggest that both mRNAs contain a long common sequence, complementary to at least two different sites on the Ad2 genome remote from the start of these two genes. Expand
A novel method to map transcripts: Evidence for homology between an adenovirus mRNA and discrete multiple regions of the viral genome
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The high sensitivity of this technique, achieved through the use of 32P-labeled RNA of high specific activity, has led to the observation that hybridization of Ad2+ND1 RNA occurs at several locations on the Ad2 genome, in addition to the expected sites of hybridization proximal to the SV40 insertion. Expand
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TLDR
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TLDR
RNA labeled with [methyl-3H] methionine and [14C]uridine was isolated from the cytoplasm of adenovirus-infected cells and purified by poly(U)-Sepharose chromatography and hybridization to filters containing immobilized adeovirus DNA suggested that the major mRNA species were methylated. Expand
One predominant 5′-undecanucleotide in adenovirus 2 late messenger RNAs
TLDR
Oligonucleotides containing the 5' termini of adenovirus 2 mRNA are selectively retained on columns of dihydroxyboryl cellulose, which was unexpected and its significance is discussed. Expand
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TLDR
The results suggest that both molecular ends serve as origins and termini for replication of adenovirus type 2 DNA. Expand
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