An alternative fixation-processing method for preembedding ultrastructural immunocytochemistry of cytoplasmic antigens: the GBS (glutaraldehyde-borohydride-saponin) procedure.


The use and properties of an alternative fixation procedure for ultrastructural cytochemistry is described that utilized primary fixation of cells with glutaraldehyde with considerable latitude in concentration and time of fixation. This was followed by treatment with sodium borohydride (as described by Weber, Rathke and Osborn (Proc Natl Acad Sci USA 75… (More)


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