An alpha-L-fucosidase from Thermus sp. with unusually broad specificity.

@article{Eneyskaya2001AnAF,
  title={An alpha-L-fucosidase from Thermus sp. with unusually broad specificity.},
  author={Elena V. Eneyskaya and Anna A Kulminskaya and Nisse Kalkkinen and Nikolay E Nifantiev and Nilkolay P. Arbatskii and Anna I. Saenko and Olga V. Chepurnaya and Alexander V. Arutyunyan and Konstantin A. Shabalin and Kirill N. Neustroev},
  journal={Glycoconjugate journal},
  year={2001},
  volume={18 10},
  pages={827-34}
}
An alpha-L-fucosidase (E.C. 3.2.1.51) exhibiting a wide aglycon specificity expressed in ability of cleaving alpha1 --> 6-, alpha1 -->3-, alpha1 --> 4-, and alpha1 --> 2-O-fucosyl bonds in fucosylated oligosaccharides, has been isolated from culture filtrate of Thermus sp. strain Y5. The alpha-L-fucosidase hydrolyzes p-nitrophenyl alpha-L-fucopyranoside with V(max) of 12.0 +/- 0.1 microM/min/mg and K(m) = 0.20 +/- 0.05 mM and is able to cleave off about 90% of total L-fucose from pronase… CONTINUE READING