An algorithm for automated detection, localization and measurement of local calcium signals from camera-based imaging.

@article{Ellefsen2014AnAF,
  title={An algorithm for automated detection, localization and measurement of local calcium signals from camera-based imaging.},
  author={Kyle L Ellefsen and Brett Settle and Ian Parker and Ian F Smith},
  journal={Cell calcium},
  year={2014},
  volume={56 3},
  pages={147-56}
}
Local Ca(2+) transients such as puffs and sparks form the building blocks of cellular Ca(2+) signaling in numerous cell types. They have traditionally been studied by linescan confocal microscopy, but advances in TIRF microscopy together with improved electron-multiplied CCD (EMCCD) cameras now enable rapid (>500 frames s(-1)) imaging of subcellular Ca(2+) signals with high spatial resolution in two dimensions. This approach yields vastly more information (ca. 1 Gb min(-1)) than linescan… CONTINUE READING
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