An aldose reductase homolog from the resurrection plant Xerophyta viscosa Baker

@article{Mundree2000AnAR,
  title={An aldose reductase homolog from the resurrection plant Xerophyta viscosa Baker},
  author={Sagadevan G. Mundree and Anne Whittaker and Jennifer Ann Thomson and Jill M. Farrant},
  journal={Planta},
  year={2000},
  volume={211},
  pages={693-700}
}
An aldose reductase homologue (ALDRXV4) was cloned from the resurrection plant Xerophyta viscosa Baker using complementation by functional sufficiency in Escherichia coli. A cDNA library constructed from X. viscosa leaves dehydrated to 85%, 37% and 5% relative water contents (RWC) was converted into an infective phagemid library. Escherichia coli (srl::Tn10) cells transformed with ds-pBluescript phagemids were selected on minimal medium plates supplemented with 1 mM isopropyl β-d… CONTINUE READING