An adaptive mutation in NS2 is essential for efficient production of infectious 1b/2a chimeric hepatitis C virus in cell culture.

@article{Chan2012AnAM,
  title={An adaptive mutation in NS2 is essential for efficient production of infectious 1b/2a chimeric hepatitis C virus in cell culture.},
  author={Katie K H Chan and Guofeng Cheng and Rudolf K. F. Beran and Huiling Yang and Todd C. Appleby and Maria V Pokrovskii and Hongmei Mo and WeiDong Zhong and William E. Delaney},
  journal={Virology},
  year={2012},
  volume={422 2},
  pages={224-34}
}
The development of JFH1 based intergenotypic recombinants which exploit the unique replication characteristics of JFH1 has made it possible to study infectious HCV encoding the structural genes of additional HCV genotypes including genotype 1b. Although, intergenotypic 1b/2a chimeric genomes replicate efficiently in transfected cells they produce very low viral titers, limiting the utility of this system. Here, intergenotypic 1b/2a variants were generated by serially passaging the virus in a… CONTINUE READING
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